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tsaC [2019-08-12 10:04:23]
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tsaC [2019-08-12 10:04:23]

L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)
Locus
BSU_36950
Isoelectric point
5.82
Molecular weight
36.85 kDa
Protein length
346 aa Sequence Blast
Gene length
1041 bp Sequence Blast
Function
tRNA modification
Product
L-threonylcarbamoyl-AMP synthase
Essential
no
E.C.
2.7.7.87
Synonyms
ipc-29d,ywlC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → Newly identified competence genes
  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    3,792,969 → 3,794,009

    Phenotypes of a mutant

  • poor growth PubMed
  • poorly transformable PubMed
  • restores viability of a smc mutant on rich medium due to the induction of a stringent response PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products PubMed
  • biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding E. coli protein YrdC) PubMed

    • Protein family

  • SUA5 family (single member, according to UniProt)

    • Structure

  • 1HRU (the homolog from E. coli) PubMed

    • Expression and Regulation

    Operons

    Genes
    tsaC
    Description
    PubMed

    Regulatory mechanism

  • DnaA: repression, PubMed, in DnaA regulon
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    Biological materials

    Mutant

  • MGNA-A195 (ywlC::erm), available at the NBRP B. subtilis, Japan
  • BKE36950 (ΔtsaC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTGACCAAACTCCTT, downstream forward: _UP4_TGAAAGCAGTCAGATGTTCT
  • BKK36950 (ΔtsaC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTGACCAAACTCCTT, downstream forward: _UP4_TGAAAGCAGTCAGATGTTCT

    • References

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